Bieker Lab - Publications


Selected publications (out of 78):


M.N. Gnanapragasam and J.J. Bieker, Orchestration of late events in erythropoiesis by KLF1/EKLF, Current Opinion in Hematology, 24, 183-190 (2017).

K.R. Gillinder, M.D. Ilsley, D. Nébor, R. Sachidanandam, M. Lajoie, G.W. Magor, M.R. Tallack, T. Bailey, M.J. Landsberg, J.P. Mackay, M.W. Parker, L.A. Miles, J.H. Graber, L.L. Peters, J.J. Bieker, A.C. Perkins, Promiscuous DNA-binding of a mutant zinc finger protein corrupts the transcriptome and diminishes cell viability, Nucleic Acids Research, 45, 1130-1143 (2017).

A. Planutis, L. Xue, C.D. Trainor, M. Dangeti, K. Gillinder, M. Siatecka, L.L. Peters, A.C. Perkins, J.J. Bieker, Neomorphic effects of the neonatal anemia (Nan-EKLF) mutation contribute to deficits throughout development, Development, 144, 430-440 (2017). [highlighted as an In this Issue preview; highlighted in Hematopoiesis News]


I.A. Aimola, H.M. Inuwa, A.J. Nok, A.I. Mamman, and J.J. Bieker, Cis-vaccenic acid induces differentiation and up-regulates gamma globin synthesis in K562, JK1, and transgenic mice erythroid progenitor stem cells, European Journal of Pharmacology, 776, 9-18 (2016). [highlighted in Hematopoiesis News]

A. Perkins, X. Xu, D.R. Higgs, G.P. Patrinos, L. Arnaud, J.J. Bieker, and S. Philipsen, “Krüppeling” erythropoiesis: an unexpected broad spectrum of human red blood cell disorders due to KLF1 variants, Blood, 127, 1856-1862 (2016). [highlighted in Hematopoiesis News]

M.N. Gnanapragasam, K.E. McGrath, S. Catherman, L. Xue, J. Palis, and J.J. Bieker, EKLF/KLF1-regulated cell cycle exit is essential for erythroblast enucleation, Blood, 128, 1631-1641 (2016). [highlighted in Hematopoiesis News]


M. Siatecka, S. Soni, A. Planutis, and J.J. Bieker, Transcriptional activity of EKLF/KLF1 modulated by PIAS3, Journal of Biological Chemistry, 290, 9929-9940 (2015).

F. Lohmann*, M. Dangeti*, S. Soni, X. Chen, A. Planutis, M.H. Baron, K. Choi, and J.J. Bieker, The DEK oncoprotein is a critical component of the EKLF/KLF1 enhancer in erythroid cells, Molecular and Cellular Biology, 35, 3726-3738 (2015). [*co-first authors] [highlighted in Experimental Hematology 43, 827 (15)]

Y.Y. Yien*, M.N. Gnanapragasam*, R. Gupta, S. Rivella, and J.J. Bieker, Alternative splicing of EKLF/KLF1 in murine primary erythroid tissues, Experimental Hematology, 43, 65-70 (2015). [*co-first authors]


L. Xue, M. Galdass, M.N. Gnanapragasam, D. Manwani, and J.J. Bieker, Extrinsic and intrinsic control by EKLF (KLF1) within a specialized erythroid niche, Development, 141, 2245-2254 (2014).

D. Manwani and J.J. Bieker, KLF1: when less is more, Blood, 124, 672-673 (2014).

S. Soni , N. Pchelintsev , P.D. Adams, and J.J. Bieker, Transcription factor EKLF (KLF1) recruitment of the histone chaperone HIRA is essential for ß-globin gene expression, Proceedings of the National Academy of Sciences, 111, 13337-13342 (2014). [highlighted in Hematopoiesis News]


J.A. Jaffray, W.B. Mitchell, M.N. Gnanapragasam, S.V. Seshan, X. Guo, C.M. Westoff, J.J. Bieker, and D. Manwani, Erythroid Transcription Factor EKLF/KLF1 Mutation Causing Congenital Dyserythropoietic Anemia Type IV in a Patient of Taiwanese Origin: Review of all reported cases and development of a clinical diagnostic paradigm, Blood Cells, Molecules, and Diseases, 51, 71-75 (2013).

Y. Yien and J.J. Bieker, EKLF/KLF1: a tissue-restricted integrator of transcriptional control, chromatin remodeling, and lineage determination, Molecular and Cellular Biology, 33, 4-13 (2013).


Y. Yien and J.J. Bieker, Functional interactions between Erythroid Krüppel-like Factor (EKLF/KLF1) and protein phosphatase PPM1B/PP2Cß, Journal of Biological Chemistry, 287, 15193-15204 (2012).


C. Mas, M. Lussier-Price, S. Soni, T. Morse, G. Arseneault, P. Di Lello, J. Lafrance-Vanasse, J.J. Bieker, and J. Omichinski, Structural and Functional Characterization of an Atypical Activation Domain in Erythroid Krüppel Like Factor (EKLF), Proceedings of the National Academy of Sciences, 108, 10484-10489 (2011).

M. Siatecka and J.J. Bieker, The multifunctional role of EKLF/KLF1 during erythropoiesis, Blood, 118, 2044-2054 (2011).


J. Chen, K.R. Peterson, C. Iancu-Rubin, and J.J Bieker, Design of embedded chimeric peptide nucleic acids that efficiently enter and accurately reactivate gene expression in vivo, Proceedings of the National Academy of Sciences, 107, 16846-16851 (2010). [highlighted in Sickle Cell News; highlighted in SciBX]

M. Siatecka, K.E. Sahr, S.G. Andersen, M. Mezei, J.J. Bieker*, and L.L Peters*, Severe anemia in the Nan mutant mouse caused by sequence-selective disruption of erythroid Krüppel-like factor, Proceedings of the National Academy of Sciences, 107, 15151-15156 (2010). [*co-corresponding authors] [highlighted in Hematopoiesis News]

J.J. Bieker, Putting a finger on the switch, Nature Genetics, 42, 733-734 (2010).

M. Siatecka, F. Lohmann, S. Bao, and J. J. Bieker, EKLF directly activates the p21WAF1/CIP1 gene by proximal promoter and novel intronic regulatory regions during erythroid differentiation, Molecular and Cellular Biology, 30, 2811-2822 (2010).

S. Schoenfelder, T. Sexton, l. Chakalova, N.F. Cope, A. Horton, S. Andrews, S. Kurukuti, J.A. Mitchell, D. Umlauf, D.S. Dimitrova, C.H. Eskiw, Y. Luo, C.L. Wei, Y. Ruan, J.J. Bieker, and P. Fraser, Preferential associations between co-regulated genes reveal a transcriptional interactome in erythroid cells, Nature Genetics, 42, 53-61 (2010).


T. Sengupta, N. Cohet, F. Morle, and J. J. Bieker, Distinct modes of gene regulation by a cell-specific transcriptional activator, Proceedings of the National Academy of Sciences, 106, 4213-4218 (2009).


T. Sengupta, K. Chen, E. Milot, and J. J. Bieker, Acetylation of EKLF is essential for epigenetic modification and transcriptional activation of the ß-globin locus, Molecular and Cellular Biology, 28, 6160-6170 (2008).

J. J. Bieker, Blood group antigens reveal their maker, Blood, 112, 1554-1555 (2008).

F. Lohmann and J. J. Bieker, Activation of Eklf expression during hematopoiesis by Gata2 and Smad5 prior to erythroid commitment, Development, 135, 2071-2082 (2008). [highlighted as an In this Issue preview]

K. J. Quadrini, E. Gruzglin, and J. J. Bieker, Non-random subcellular distribution of variant EKLF in erythroid cells, Experimental Cell Research, 314, 1595-1604 (2008).

D. Manwani and J. J. Bieker, The Erythroblastic Island, Current Topics in Developmental Biology, 82, 23-53 (2008).


M. P. Frontelo*, D. Manwani*, M. Galdass, H. Karsunky, F. Lohmann, P. G. Gallagher, and J. J. Bieker, Novel role for EKLF in megakaryocyte lineage commitment, Blood, 110, 3871-3880 (2007). [*co-first authors] [highlighted as an Inside Blood preview]

M. Siatecka, L. Xue, and J. J. Bieker, Sumoylation of EKLF Promotes Transcriptional Repression and Is Involved in Inhibition of Megakaryopoiesis, Molecular and Cellular Biology, 27, 8547-8560 (2007).

D. Manwani. M. Galdass, and J. J. Bieker, Altered Regulation of ß-like Globin Genes by a Redesigned Erythroid Transcription Factor, Experimental Hematology, 35, 39-47 (2007).


K. J. Quadrini and J. J. Bieker, EKLF/KLF1 is Ubiquitinated and its Stability is Regulated by Activation Domain Sequences Through the 26S Proteasome, FEBS Letters, 580, 2285-2293 (2006).


J. J. Bieker, Probing the Onset and Regulation of Erythroid Cell-Specific Gene Expression, Mount Sinai Journal of Medicine, 72, 333-338 (2005). 

J. J. Bieker, An unexpected entry into the globin real estate market, Blood, 106, 2230-2231 (2005).

H. Im, J. A. Grass, K. D. Johnson, S. Kim, M. E. Boyer, A. N. Imbalzano, J. J. Bieker, and E. H. Bresnick, Chromatin Domain Activation Via GATA-1 Utilization of a Small Subset of Dispersed GATA Motifs Within a Broad Chromosomal Region, Proc. Natl. Acad. Sci., 102, 17065-17070 (2005).


L. Xue, X. Chen, Y. Chang, and J. J. Bieker, Regulatory Elements of the EKLF Gene That Direct Erythroid Cell-specific Expression During Mammalian Development, Blood, 103, 4078-4083 (2004).

X. Chen and J. J. Bieker, Stage-specific Repression by the EKLF Transcriptional Activator, Molecular and Cellular Biology, 24, 10416-10424 (2004).


C. A. Adelman, S. Chattopadyay, and J. J. Bieker, The BMP/BMPR/Smad Pathway Directs Expression of the Erythroid-Specific EKLF and GATA1 Transcription Factors During Embryoid Body Differentiation in Serum-free Media, Development, 129, 539-549 (2002).

K. J. Quadrini and J. J. Bieker, Krüppel-like Zinc Fingers Bind to Nuclear Import Proteins and are Required for Efficient Nuclear Localization of EKLF, Journal of Biological Chemistry, 277, 32243-32252 (2002).


W. Zhang, S. Kadam, B. M. Emerson, and J. J. Bieker, Site-Specific Acetylation by p300/CBP Regulates Erythroid Krüppel-like Factor (EKLF) Transcriptional Activity via its Interaction with the SWI/SNF Complex, Molecular and Cellular Biology, 21, 2413-2422 (2001).

X. Chen and J. J. Bieker, Unanticipated Repression Function Linked to Erythroid Krüppel-like Factor (EKLF), Molecular and Cellular Biology, 21, 3118-3125 (2001).

J. J. Bieker, Krüppel-like Factors: Three Fingers in Many Pies, J. Biol. Chem., 276, 34355-34358 (2001).


  1. J.J. Bieker, EKLF and the development of the erythroid lineage, in Transcription Factors: Normal and Malignant Development of Blood Cells  (ed. K. Ravid and J. Licht) (2000).

For a complete list of published work please click here.